Geochemical signatures were highly distinct among rivers, with an average classification accuracy of 93% using only those variables where otolith values were accurately predicted from water chemistry data. While Sr:Ca, Ba:Ca, 87Sr: 86Sr, and δ 18O values in otoliths reflected those ratios in ambient waters, Mg:Ca and Mn:Ca ratios in otoliths varied independently of water chemistry. We examined correlations between water chemistry and otolith composition for five rivers where both were sampled. ![]() Otoliths and (or) water samples were collected from 20 major spawning rivers from Florida to Quebec and were analyzed for elemental (Mg:Ca, Mn:Ca, Sr:Ca, and Ba:Ca) and isotope ( 87Sr: 86Sr and δ 18O) ratios. ![]() ![]() We assembled a comprehensive atlas of geochemical signatures in juvenile American shad ( Alosa sapidissima) to discriminate natal river origins on a large spatial scale and at a high spatial resolution.
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